Giovedì 6 luglio ore 15 presso la sala conferenze del TBMLab in Via Garofalo, 39 (Milano) il Dr. Bradley Layton della Drexel University, in occasione di una sua visita al NITLab terrà un breve seminario sulla sua ricerca corrente.
I contenuti del seminario sono i seguenti:
There is an urgent need to develop high-precision, high-throughput instrumentation for understanding the fundamental aspects of neurological diseases associated with the cytoskeleton and its motor proteins. Our broad long-term objective is to develop a robust high-throughput protocol for measuring mechanical forces generated by specific molecular motors of the axonal cytoskeleton and in the extracellular matrix protein collagen. The cytoskeleton has been implicated in a vast array of neurological diseases such as ALS, Parkinson’s, epilepsy, neuropathy, and neuron-based cancers. Our objective is to introduce a highly parallel nanoscale force transduction device to the scientific community, to test the hypotheses regarding the amount and distribution of actin filaments and microtubules along with their associated motor proteins, myosin-II, cytoplasmic dynein, and Eg5. The working hypothesis for this aim is that we will be able to develop and refine a novel method using a microfabricated polymeric microbrush array to simultaneously but individually measure tensile forces on multiple axons in culture, and then confirm the efficacy of this approach by quantifying the diminution of tension in the axon associated with actin depolymerization and the increase in tension associated with microtubule depolymerization.
The talk will also focus on recent progress on isolating a novel collagen from the marine cyanobacteria, Trichodesmium erythraeum, as well as progress on developing a microfabricated hematology device to be used aboard spacecraft.
Ecco un breve CV del Dr.Layton
Dr. Bradley Layton received his PhD in Biomedical Engineering from the University of Michigan in 2003, and completed two post-doctoral fellowships in neurology and in radiology. Dr. Layton holds a BS in mechanical engineering from MIT and an MS in mechanical engineering from the University of Michigan. Prior to graduate school Dr. Layton designed and built titanium bicycle frames and Kevlar wakeless boats, developed software for the BMDO, evaluated properties of lunar soil for the DOE and NASA, he inspected radioactive containment buildings at the Savannah River Site, and designed equipment for Northrop Grumman and the USEPA.
Dr. Layton is working closely with his colleagues at Drexel’s three main campuses to: (1) develop a molecular-mechanics based model for aggregation limits in collagen (2) develop a nanoscale single protein testing system to examine the deformation characteristics of proteins at both the quaternary and supraquatenary scales (3) probe membrane and cell responses to mechanical stimuli for the purpose of quantifying and potentially reducing wound and injury recovery times and for developing cell-based sensors, (4) better understand how slight alterations in genomes can account for drastic differences in behavior and capabilities among similar species, and to (5) develop a method for sorting astronaut blood cells in space.
Dr. Layton teaches graduate engineering mathematics, undergraduate engineering design and MechanoEvolution. Dr. Layton’s 2004-2005 freshman design group received the Best Freshman Design Project Award for their project entitled “How to Build Your own Atomic Force Microscope.” He was also recognized by Pennsylvania Governor Ed Rendell for his leadership in preparing a local middle school robotics team prepare for a robotics competition in Japan.
Dr. Layton currently has publications in the ASME Biomechanical Engineering Journal, BioTechniques, Diabetes Metabolism Research and Reviews, Journal of Engineering Materials and Technology, Journal of Biomechanics, Acta Biomaterilia and MicroElectronics Journal.
He is a member and contributor to the ASME, BMES, and IEEE-EMBS. He has funding from NSF, NASA and the State of Pennsylvania.